Journal: Experimental and Therapeutic Medicine

Article Title: Negative pressure wound therapy enhances bone regeneration compared with conventional therapy in a rabbit radius gap-healing model

doi: 10.3892/etm.2021.9905

Figure Lengend Snippet: VEGF, BMP-2 and OPN protein expression. (A) Representative western blot image and quantification of (B) VEGF, (C) BMP-2 and (D) OPN protein expression at each time point examined in the NPWT and control groups. * P<0.05 and ** P<0.01. VEGF, vascular endothelial growth factor; BMP-2, bone morphogenetic protein 2; OPN, osteopontin; NPWT, negative pressure wound therapy; 2W, week 2; 4W, week 4.

Article Snippet: The membranes were then incubated overnight at 4˚C with TBS-Tween-20 supplemented with one of the following primary antibodies: Mouse anti-β-actin (1:1,000; Boster Biological Technology; cat. no. P60709), mouse anti-VEGF (1:200; Santa Cruz Biotechnology, Inc. cat. no. sc-7269), mouse anti-BMP-2 (1:400; Santa Cruz Biotechnology, Inc. cat. no. sc-137087) or mouse anti-OPN (1:400; Abcam; cat. no. ab228748).

Techniques: Expressing, Western Blot

Journal: BMC Musculoskeletal Disorders

Article Title: Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis

doi: 10.1186/s12891-023-06339-4

Figure Lengend Snippet: Expression ratios of mRNA in the synovial membrane

Article Snippet: Indirect immunohistochemical techniques (with paraffin peroxidase) were used in all the biopsies, in which the mouse monoclonal anti-BMP2 ab6285 antibody (clone 65529.111) of Abcam® and rabbit monoclonal anti-Dkk1 antibody were used as the primary antibodies. ab109416 (clone EPR4759) from Abcam®, rabbit anti-BMP2 monoclonal antibody ab 124,715 (clone EPR6211) from Abcam®, mouse monoclonal anti-Wnt5a ab86720 (clone 3D10) from Abcam®, mouse monoclonal antibody anti-TGF-β1 ab64715 (clone 2Ar2) from Abcam®, Abcam® ab79056 rabbit anti-IL-17 A polyclonal antibody, and Dako® K-5007 antibody (EnVision TM Detection Systems Peroxidase / DAB), were used as the secondary antibodies.

Techniques: Expressing

Journal: BMC Musculoskeletal Disorders

Article Title: Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis

doi: 10.1186/s12891-023-06339-4

Figure Lengend Snippet: Correlation between gene expression of IL-17 A with IL-23, Dkk1, BMP2, and BMP4

Article Snippet: Indirect immunohistochemical techniques (with paraffin peroxidase) were used in all the biopsies, in which the mouse monoclonal anti-BMP2 ab6285 antibody (clone 65529.111) of Abcam® and rabbit monoclonal anti-Dkk1 antibody were used as the primary antibodies. ab109416 (clone EPR4759) from Abcam®, rabbit anti-BMP2 monoclonal antibody ab 124,715 (clone EPR6211) from Abcam®, mouse monoclonal anti-Wnt5a ab86720 (clone 3D10) from Abcam®, mouse monoclonal antibody anti-TGF-β1 ab64715 (clone 2Ar2) from Abcam®, Abcam® ab79056 rabbit anti-IL-17 A polyclonal antibody, and Dako® K-5007 antibody (EnVision TM Detection Systems Peroxidase / DAB), were used as the secondary antibodies.

Techniques: Gene Expression

Journal: BMC Musculoskeletal Disorders

Article Title: Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis

doi: 10.1186/s12891-023-06339-4

Figure Lengend Snippet: Differences between gene expression of IL-17 A, IL-23, Dkk1, TGF-β1, BMP2, and BMP4 SpA: spondyloarthritis (n= 18): RA: rheumatoid arthritis (n=8); OA: osteoarthritis (n=16) (*) p= 0.001

Article Snippet: Indirect immunohistochemical techniques (with paraffin peroxidase) were used in all the biopsies, in which the mouse monoclonal anti-BMP2 ab6285 antibody (clone 65529.111) of Abcam® and rabbit monoclonal anti-Dkk1 antibody were used as the primary antibodies. ab109416 (clone EPR4759) from Abcam®, rabbit anti-BMP2 monoclonal antibody ab 124,715 (clone EPR6211) from Abcam®, mouse monoclonal anti-Wnt5a ab86720 (clone 3D10) from Abcam®, mouse monoclonal antibody anti-TGF-β1 ab64715 (clone 2Ar2) from Abcam®, Abcam® ab79056 rabbit anti-IL-17 A polyclonal antibody, and Dako® K-5007 antibody (EnVision TM Detection Systems Peroxidase / DAB), were used as the secondary antibodies.

Techniques: Gene Expression

Journal: BMC Musculoskeletal Disorders

Article Title: Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis

doi: 10.1186/s12891-023-06339-4

Figure Lengend Snippet: Differences between gene expression of IL-17 A, IL-23, Dkk1, TGF-β1, BMP2, and BMP4 (excluding ankylosing spondylitis patients) PsA: psoriatic arthritis (n= 14); RA: rheumatoid arthritis (n=8); OA: osteoarthritis (n=16) (*) p= 0.018

Article Snippet: Indirect immunohistochemical techniques (with paraffin peroxidase) were used in all the biopsies, in which the mouse monoclonal anti-BMP2 ab6285 antibody (clone 65529.111) of Abcam® and rabbit monoclonal anti-Dkk1 antibody were used as the primary antibodies. ab109416 (clone EPR4759) from Abcam®, rabbit anti-BMP2 monoclonal antibody ab 124,715 (clone EPR6211) from Abcam®, mouse monoclonal anti-Wnt5a ab86720 (clone 3D10) from Abcam®, mouse monoclonal antibody anti-TGF-β1 ab64715 (clone 2Ar2) from Abcam®, Abcam® ab79056 rabbit anti-IL-17 A polyclonal antibody, and Dako® K-5007 antibody (EnVision TM Detection Systems Peroxidase / DAB), were used as the secondary antibodies.

Techniques: Gene Expression

Journal: BMC Musculoskeletal Disorders

Article Title: Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis

doi: 10.1186/s12891-023-06339-4

Figure Lengend Snippet: IHC reactivity differences according to the diseases

Article Snippet: Indirect immunohistochemical techniques (with paraffin peroxidase) were used in all the biopsies, in which the mouse monoclonal anti-BMP2 ab6285 antibody (clone 65529.111) of Abcam® and rabbit monoclonal anti-Dkk1 antibody were used as the primary antibodies. ab109416 (clone EPR4759) from Abcam®, rabbit anti-BMP2 monoclonal antibody ab 124,715 (clone EPR6211) from Abcam®, mouse monoclonal anti-Wnt5a ab86720 (clone 3D10) from Abcam®, mouse monoclonal antibody anti-TGF-β1 ab64715 (clone 2Ar2) from Abcam®, Abcam® ab79056 rabbit anti-IL-17 A polyclonal antibody, and Dako® K-5007 antibody (EnVision TM Detection Systems Peroxidase / DAB), were used as the secondary antibodies.

Techniques:

Journal: BMC Musculoskeletal Disorders

Article Title: Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis

doi: 10.1186/s12891-023-06339-4

Figure Lengend Snippet: Serum levels of proteins according to the diseases

Article Snippet: Indirect immunohistochemical techniques (with paraffin peroxidase) were used in all the biopsies, in which the mouse monoclonal anti-BMP2 ab6285 antibody (clone 65529.111) of Abcam® and rabbit monoclonal anti-Dkk1 antibody were used as the primary antibodies. ab109416 (clone EPR4759) from Abcam®, rabbit anti-BMP2 monoclonal antibody ab 124,715 (clone EPR6211) from Abcam®, mouse monoclonal anti-Wnt5a ab86720 (clone 3D10) from Abcam®, mouse monoclonal antibody anti-TGF-β1 ab64715 (clone 2Ar2) from Abcam®, Abcam® ab79056 rabbit anti-IL-17 A polyclonal antibody, and Dako® K-5007 antibody (EnVision TM Detection Systems Peroxidase / DAB), were used as the secondary antibodies.

Techniques:

Journal: eLife

Article Title: Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome

doi: 10.7554/eLife.54383

Figure Lengend Snippet: Heat-map representing DE genes between reprogrammed vascular cells from young (N = 3) vs. old (N = 3) donors (iSMCs ( A ), iVECs ( B )). Z score = ± 1.5. DE genes with log 2 FC > 1 and FDR < 0.05. Quantification of BMP2 ( C ) and PALD1 ( D ) expression in human skin biopsies from young vs. old donors (N = 2 donors per condition; N = 10 tissue sections per condition; Student’s t-test with p<0.001 (***) and p<0.05 (*)). Representative images of BMP2 ( E ) and PALD1 ( F ) from skin biopsies obtained from young vs. old donors. Scale bars: 50 µm. Quantification of vascular permeability using young vs. old reprogrammed cells ( G–J ). Schematic showing the generation of an endothelial monolayer covering the interface with a 3D matrix embedding SMCs. Vascular permeability was quantified by measuring the variation of 70 kDa dextran fluorescent intensity across the interface ( G ). Representative images of dextran flow (red) through the endothelial monolayer in presence of young vs. old reprogrammed cells. Scale bar: 100 µm ( H ). Quantification of vascular permeability in presence of young vs. old iVECs (I, at least N = 40 independent measurements per condition in three biological replicates; ANOVA with Holm-Sidak test; comparison with primary VECs (** is p<0.01 and *** is p<0.001); comparison with young iVECs (♦♦♦is p<0.001); comparison with old iVECs (••• is p<0.001)) or young vs. old iSMCs (J, at least N = 40 independent measurements per condition in three biological replicates; ANOVA with Holm-Sidak test; * is p<0.05, ** is p<0.01 and *** is p<0.001). Quantification of vascular permeability in presence of PALD1 KD ( K ) or BMP2 KD ( L ) in primary VECs and SMCs, respectively (at least N = 60 independent measurements per condition in three biological replicates for each KD experiment; Student's t-test; *** is p<0.001).

Article Snippet: Antibody , Anti-human BMP2 (Rabbit polyclonal) , Novus , Cat#nBP1-19751 RRID: AB_2227877 , IF(1:100).

Techniques: Expressing, Permeability, Comparison

Journal: eLife

Article Title: Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome

doi: 10.7554/eLife.54383

Figure Lengend Snippet: Note that BMP2 KD was validated using qPCR due to technical problems with any of the tested anti-BMP2 antibodies (Western Blot). The use of these antibodies resulted in multiple aspecific bands.

Article Snippet: Antibody , Anti-human BMP2 (Rabbit polyclonal) , Novus , Cat#nBP1-19751 RRID: AB_2227877 , IF(1:100).

Techniques: Western Blot

Journal: eLife

Article Title: Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome

doi: 10.7554/eLife.54383

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-human BMP2 (Rabbit polyclonal) , Novus , Cat#nBP1-19751 RRID: AB_2227877 , IF(1:100).

Techniques: Transfection, Construct, Retroviral, Expressing, Isolation, Clinical Proteomics, Blocking Assay, Sequencing, Enzyme-linked Immunosorbent Assay, Software

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: FBS-Derived Exosomes as a Natural Nano-Scale Carrier for Icariin Promote Osteoblast Proliferation

doi: 10.3389/fbioe.2021.615920

Figure Lengend Snippet: Effects of ICA on osteoblast proliferation. (A) ICA (0.1 μg/mL) promoted osteoblast proliferation; (B) Time dependence of the effect of ICA on osteoblast proliferation activity; and (C) Increased protein expression of BMP-2, RUNX2, and OPN after ICA was applied to MC3T3-E1 cells. * P -value < 0.05 ( n = 4).

Article Snippet: The membranes were incubated overnight at 4°C with specific anti-CD63 (diluted 1: 200; Abcam, United States), anti-CD81 (diluted 1: 500; Abcam, United States), anti-CD40 (diluted 1: 1000; Bioss, China), anti-ALIX (diluted 1: 1000; Abbexa, United Kingdom), anti-RUNX2 (diluted 1: 500, SAB, United States), anti-BMP-2 (diluted 1: 500, Bioworld, United States), anti-OPN (diluted 1: 1000, Proteintech, United States), and anti-GAPDH (diluted 1: 5000, Bioworld, United States).

Techniques: Activity Assay, Expressing